Podocarpo, a plant of ornamental interest: asexual propagation

MATERIAL AND METHODS Two experiments were carried out in the sequence, both developed at the Protected Cultivation and Biological Control Station "Professor Doctor Mário Cesar Lopes", belonging to the Experimental Station of Horticulture and Protected Culture of Unioeste, Campus Marechal Cândi-do Rondon (PR), under geographic coordinates of latitude 24°33'22"S and longitude 54°03'24"W, and altitude of approximately 400 m. Both experiments were conducted under screen conditions with 50% shading. For the first experiment, the experimental design was a randomized block design with a 4 x 3 x 2 factorial design [0, 500, 1000 and 1500 mg L-1 of indolebutyric acid (IBA) x 4, 6 and 8 x 2-leaf cuttings (end of winter-August/2017 and summer-January/2018)], resulting in 24 treatments, containing 3 replicates and 15 cuttings per replicate, totaling 1080 cuttings. The matrix plants were purchased from ornamental plant producers in the municipality of Marechal Cândido Rondon, transplanted to 12 L polyethylene containers containing a mixture of latosol, sand and organic matter (1:1:1, v/v/v), acclimatized in nursery for 90 days and with relevant cultural practices (fertilization, irrigation and pest and disease care). For the preparation of the solutions containing IBA, IAA and NAA, each of the phytoregulators to be tested according to the previously established treatments were weighed into an analytical precision balance, followed by slow immersion, dilution of the phytoregu-lators in 0.25 L of ethyl alcohol hydrated at 46.2 ° G.L., the volumes were filled with water to 0.5 L after complete dilution and for quick immersion the dilution of the phytoregulators occurred in 0.1 L of the same alcohol as above, with volume subsequently supplemented with water to 0.2 L after the total dilution.