Adaptive control of the E. coli-specific growth rate in fed-batch cultivation based on oxygen uptake rate

In this study, an automatic control system is developed for the setpoint control of the cell biomass specific growth rate (SGR) in fed-batch cultivation processes. The feedback signal in the control system is obtained from the oxygen uptake rate (OUR) measurement-based SGR estimator. The OUR online measurements adapt the system controller to time-varying operating conditions. The developed approach of the PI controller adaptation is presented and discussed. The feasibility of the control system for tracking a desired biomass growth time profile is demonstrated with numerical simulations and fed-batch culture E.coli control experiments in a laboratory-scale bioreactor. The procedure was cross-validated with the open-loop digital twin SGR estimator, as well as with the adaptive control of the SGR, by tracking a desired setpoint time profile. The digital twin behavior statistically showed less of a bias when compared to SGR estimator performance. However, the adaptation—when using first principles—was outperformed 30 times by the model predictive controller in a robustness check scenario. •The adaptive control of specific growth rate (SGR) relies only upon the oxygen uptake rate in growth-limiting fed-batch bioprocesses.•The adaptation is based on the controlled process's first principles-based tendency model.•The procedure was cross-validated with an open-loop digital twin, a closed-loop SGR estimator, and model predictive control behavior.