Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases

Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases

The heterogeneity of tumor infiltrating lymphocytes (TILs) is not well characterized in brain metastasis. To address this, we performed a targeted analysis of immune-cell subsets in brain metastasis tissues to test immunosuppressive routes involved in brain metastasis. We performed multiplex immunof...

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Veröffentlicht in:Biology (Basel, Switzerland) Switzerland), 2021-05, Vol.10 (5), p.425
Hauptverfasser: Croft, Priyakshi Kalita-de, Chittoory, Haarika, Nguyen, Tam H, Saunus, Jodi M, Kim, Woo Gyeong, McCart Reed, Amy E, Lim, Malcolm, De Luca, Xavier M, Ferguson, Kaltin, Niland, Colleen, Mazzieri, Roberta, Dolcetti, Riccardo, Simpson, Peter T, Lakhani, Sunil R
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies
Antigens
Brain cancer
brain metastasis
Brain research
Brain tumors
Breast cancer
CD4 antigen
CD8 antigen
Cytokines
Genes
immune checkpoint
Immunofluorescence
Immunosurveillance
Immunotherapy
Ligands
Lymphocytes
Lymphocytes T
Metastases
Metastasis
Microenvironments
Microglia
Paraffin
Phenotyping
Software
T-cells
TILs
Tumor cells
tumor microenvironment
Tumor-infiltrating lymphocytes
Tumors
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Zusammenfassung:The heterogeneity of tumor infiltrating lymphocytes (TILs) is not well characterized in brain metastasis. To address this, we performed a targeted analysis of immune-cell subsets in brain metastasis tissues to test immunosuppressive routes involved in brain metastasis. We performed multiplex immunofluorescence (mIF), using commercially available validated antibodies on formalin-fixed paraffin embedded whole sections. We quantitated the subsets of immune-cells utilizing a targeted panel of proteins including PanCK, CD8, CD4, VISTA and IBA-1, and analyzed an average of 15,000 cells per sample. Classifying tumors as either high (>30%) or low (
ISSN:2079-7737
2079-7737
DOI:10.3390/biology10050425