Role of Cholecystokinin ( cck ) in Feeding Regulation of Largemouth Bass ( Micropterus salmoides ): Peptide Activation and Antagonist Inhibition

This study investigated the role of cholecystokinin ( ) in the feeding regulation of largemouth bass ( ) via peptide activation and antagonist inhibition. The results show that the gene was expressed in various tissues, with the highest expression level occurring in the brain. Feeding, continuous feeding, and refeeding after fasting could significantly improve the mRNA levels of in the brain. Moreover, the activation of via injecting an exogenous CCK peptide could inhibit feed intake by regulating the mRNA levels of anorexigenic and feed-promoting factors in the brain and intestine. Furthermore, the CCK peptide reduced feed intake; however, the presence of an antagonist (Ly225910-CCK1R and devazepide-CCK2R) could reverse this effect through regulating the mRNA levels of anorexigenic and feed-promoting factors in the brain and intestine. Treatment with devazepide + CCK (CCK2R) reversed feed intake more effectively than Ly225910 + CCK (CCK1R) treatment. In summary, could regulate the feed intake of largemouth bass through regulating feeding-related genes in the brain and intestine. In addition, required binding with the receptor to inhibit feed intake more effectively in largemouth bass, and the binding effect of CCK1R was better than that of CCK2R.