Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background

Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies. Resource tableImage 1Unique stem cell line identifierTUSMi002-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of MedicineContact information of distributorYing Wang, wangying_csu@126.comJing Zhang, julianzj07@163.comType of cell lineiPSCOriginHumanAdditional origin infoApplicable for human iPSCAge: 65Sex: femaleEthnicity if known:Cell sourcePeripheral blood mononuclear cells (PBMCs)Method of reprogrammingTransgene freeGenetic ModificationN/AType of ModificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemNon-integrating episomal vector systemDate archived/stock date20161110Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/2289Ethical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC.D (BG).016.01.1)