Surveillance of Coxiella burnetii Shedding in Three Naturally Infected Dairy Goat Herds after Vaccination, Focusing on Bulk Tank Milk and Dust Swabs
Q fever outbreaks on three dairy goat farms (A-C) were monitored after the animals had been vaccinated with an inactivated phase I vaccine. The antibody response was measured before vaccination by serum samples with two phase-specific ELISAs to characterize the disease status. Shedding was determine...
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Veröffentlicht in: | Veterinary sciences 2022-02, Vol.9 (3), p.102 |
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Zusammenfassung: | Q fever outbreaks on three dairy goat farms (A-C) were monitored after the animals had been vaccinated with an inactivated
phase I vaccine. The antibody response was measured before vaccination by serum samples with two
phase-specific ELISAs to characterize the disease status. Shedding was determined by vaginal swabs during three kidding seasons and monthly bulk tank milk (BTM) samples. Dust swabs from one windowsill of each barn and from the milking parlors were collected monthly to evaluate the indoor exposure. These samples were analyzed by qPCR. The phase-specific serology revealed an acute Q fever infection in herd A, whereas herds B and C had an ongoing and past infection, respectively. In all three herds, vaginal shedders were present during three kidding seasons. In total, 50%, 69%, and 15% of all collected BTM samples were
positive in herds A, B, and C, respectively. Barn dust contained
DNA in 71%, 45%, and 50% of examined swabs collected from farms A, B, and C, respectively. The largest number of
positive samples was obtained from the milking parlor (A: 91%, B: 72%, C: 73%), indicating a high risk for humans to acquire Q fever during milking activity. |
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ISSN: | 2306-7381 2306-7381 |
DOI: | 10.3390/vetsci9030102 |