Genetic variability at exon 2 of inhibin alpha locus in malabari goats of kerala

The present study included 207 Malabari goats from six centers viz., University Goat and Sheep Farm, Mannuthy and 5 field centres of the ICAR-All India Coordinated Research Project on Goat Improvement (Malabari). The study was planned to detect single nucleotide variations in inhibin alpha gene of Malabari goats. Genomic DNA was isolated from 207 animals and PCR was done to amplify 220 bp fragment of exon 2 region of inhibin alpha (INHA) gene. Single strand conformation polymorphism (SSCP) technique was used to detect single nucleotide polymorphisms (SNPs). The SSCP showed a similar pattern of two bands (CC) for all samples and the population was found to be monomorphic for the locus.