ImmunoFISH for Adherent Cultured Mammalian Cells

This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.

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Bibliographische Detailangaben
Veröffentlicht in:Bio-protocol 2013-12, Vol.3 (24)
Hauptverfasser: Rossiello, Francesca, Fumagalli, Marzia, di Fagagna, Fabrizio
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
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Zusammenfassung:This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.999