Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of sub...

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Veröffentlicht in:	Czech Journal of Food Sciences 2014-01, Vol.32 (1), p.48-53
Hauptverfasser:	Blanco, K.C., University Estadual Paulista, Rio Claro (Brazil). Dept. of Biochemistry and Microbiology, De Moraes, F.F., University Estadual de Maringa (Brazil). Chemical Engineering Dept, Bernardi, N.S., University Estadual Paulista, Rio Claro (Brazil). Dept. of Biochemistry and Microbiology, Vettori, M.H.P.B., University Estadual Paulista, Rio Claro (Brazil). Dept. of Biochemistry and Microbiology, Monti, R., University Estadual Paulista, Rio Claro (Brazil). Dept. of Biochemistry and Microbiology, Contiero, J., University Estadual Paulista, Rio Claro (Brazil). Dept. of Biochemistry and Microbiology
Format:	Artikel
Sprache:	eng
Schlagworte:	ABSORBANCE ABSORBANCIA ACTIVADOR ENZIMATICO ACTIVATEUR D'ENZYME ACTIVIDAD CATALITICA ACTIVIDAD ENZIMATICA ACTIVITE CATALYTIQUE ACTIVITE ENZYMATIQUE Affinity Affinity chromatography AGUAS RESIDUALES ALMIDON AMIDON ANALYTICAL METHODS BACILLUS Bacillus lehensis BACILO BRASIL BRAZIL BRESIL CASSAVA CATALYTIC ACTIVITY CHROMATOGRAPHIE CHROMATOGRAPHY CONTENIDO DE NITROGENO CROMATOGRAFIA Cyclodextrin cyclodextrin glycosyltransferase Cyclodextrins DEXTRANAS DEXTRANE DEXTRANS EAU USEE ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS ENZIMAS Enzymatic activity ENZYME ENZYME ACTIVATORS ENZYME ACTIVITY ENZYME INHIBITORS Enzyme kinetics ENZYMES EQUIPO DE LABORATORIO ESTABILIDAD FERMENTACION FERMENTATION Gel electrophoresis Glycosyltransferase INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME LABORATORY EQUIPMENT LOSSES MANDIOCA MANIOC MATERIEL DE LABORATOIRE MEASUREMENT MEDICION MESURE MOLECULAR WEIGHT NITROGEN CONTENT PERDIDAS PERTE PESO MOLECULAR pH effects POIDS MOLECULAIRE PROCESAMIENTO PROCESSING Protein purification PURIFICACION PURIFICATION Sodium lauryl sulfate STABILITE STABILITY STARCH TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS TEMPERATURA TEMPERATURE TEMPS TENEUR EN AZOTE TIEMPO TIME TRAITEMENT WASTEWATER
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Zusammenfassung:	The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using beta-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cyclodextrin glycosyltransferase were 55 deg C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l/h.
ISSN:	1212-1800 1805-9317
DOI:	10.17221/432/2012-CJFS