Genome-wide identification and expression analysis of the cucumber PYL gene family

Abscisic acid (ABA) is a very important hormone in plants. It regulates growth and development of plants and plays an important role in biotic and abiotic stresses. The Pyrabactin resistance 1-like (PYR/PYL) proteins play a central role in ABA signal transduction pathways. The working system of genes in cucumber, an important economical vegetable ( L.), has not been fully studied yet. Through bioinformatics, a total of 14 individual genes were identified in Chinese long '9930' cucumber. Fourteen genes were distributed on six chromosomes of cucumber, and their encoded proteins predicted to be distributed in cytoplasm and nucleus. Based on the phylogenetic analysis, the genes of cucumber, , rice, apple, and soybeancould be classified into three groups. Genetic structures and conserved domains analysis revealed that genes in the same group have similar exons and conserved domains. By predicting cis-elements in the promoters, we found that all members contained hormone and stress-related elements. Additionally, the expression patterns of genes were specific in tissues. Finally, we further examined the expression of 14 genes under ABA, PEG, salt stress. The qRT-PCR results showed that most gene expression levels were up-regulated. Furthermore, with different treatments about 3h, the relative expression of was up-regulated and more than 20 times higher than 0h. It indicated that this gene may play an important role in abiotic stress.