New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish
The diversity of O-polysaccharides (O-antigens) among 28 strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by H and C NMR spectr...
Gespeichert in:
Veröffentlicht in: | Microorganisms (Basel) 2024-08, Vol.12 (8), p.1575 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The diversity of O-polysaccharides (O-antigens) among 28
strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by
H and
C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of
O-antigen has been established: →3)-α-L-Rha
-(1→3)-α-D-Man
NAc-(1→2)-β-D-Glc
-(1→3)-α-L-Rha
2OAc4OAc-(1→3)-β-D-Man
NAc-(1→3)-α-D-Glc
-(1→. This new
O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish. |
---|---|
ISSN: | 2076-2607 2076-2607 |
DOI: | 10.3390/microorganisms12081575 |