A validated restriction enzyme ddPCR cg05575921 (AHRR) assay to accurately assess smoking exposure

In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array. The RE ddP...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinical epigenetics 2024-03, Vol.16 (1), p.45-45, Article 45
Hauptverfasser:	Fitzgerald, Sandra, Bhat, Basharat, Print, Cristin, Jones, Gregory T
Format:	Artikel
Sprache:	eng
Schlagworte:	Aryl hydrocarbon receptor repressor (AHRR) Basic Helix-Loop-Helix Transcription Factors - genetics Bisulfite Bisulfite conversion Brief Report Chronic diseases Comparative analysis DNA Methylation Droplet digital PCR Enzymes Epigenetic inheritance Epigenetics Ethylenediaminetetraacetic acid Genetic testing Humans Methylation Polymerase Chain Reaction Repressor Proteins - genetics Restriction enzyme Smokers Smoking Smoking - genetics Software Sulfites
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In this study, a novel restriction enzyme (RE) digestion-based droplet digital polymerase chain reaction (ddPCR) assay was designed for cg005575921 within the AHRR gene body and compared with matching results obtained by bisulfite conversion (BIS) ddPCR and Illumina DNA methylation array. The RE ddPCR cg05575921 assay appeared concordant with BIS ddPCR (r = 0.94, P
ISSN:	1868-7083 1868-7075 1868-7083 1868-7075
DOI:	10.1186/s13148-024-01659-1