Generation of a heterozygous FUS-Q290X knock in human embryonic stem cell line (WAe009-A-83) using CRISPR/Cas9 system

Generation of a heterozygous FUS-Q290X knock in human embryonic stem cell line (WAe009-A-83) using CRISPR/Cas9 system

Fused in Sarcoma (FUS) gene encodes FUS RNA binding protein, a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein complex, which is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm, and it has been implicated in regulation of gene ex...

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Veröffentlicht in:Stem cell research 2022-04, Vol.60, p.102734-102734, Article 102734
Hauptverfasser: Zhang, Yamin, Zhu, Jing, Dai, Yaozhang, Wang, Liuzhong, Liu, Rui, Guo, Xiao
Format: Artikel
Sprache:eng
Schlagworte:
Amyotrophic Lateral Sclerosis - metabolism
CRISPR-Cas Systems - genetics
Human Embryonic Stem Cells - metabolism
Humans
Liposarcoma - genetics
Mutation
RNA, Messenger - metabolism
RNA-Binding Protein FUS - genetics
RNA-Binding Protein FUS - metabolism
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Zusammenfassung:Fused in Sarcoma (FUS) gene encodes FUS RNA binding protein, a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein complex, which is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm, and it has been implicated in regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. FUS gene mutations result in amyotrophic lateral sclerosis and Liposarcoma. This heterozygous FUS-Q290X knock in hESC line will be a valuable tool to investigate the disease mechanisms of amyotrophic lateral sclerosis and Liposarcoma.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102734