Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock

Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock

The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. C...

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Veröffentlicht in:Journal of Genetic Engineering and Biotechnology 2015-06, Vol.13 (1), p.19-23
Hauptverfasser: Sadeghi, F., Yadollahi, A., Kermani, M. Jafarkhani, Eftekhari, M.
Format: Artikel
Sprache:eng
Schlagworte:
culture media
genotype
In vitro propagation
indole butyric acid
iron
micropropagation
Plant Biotechnology
Prunus
Rooting
rootstocks
shoots
Tetra
thiamin
Vegetative rootstock
woody plants
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Zusammenfassung:The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. Culture establishment using nodal segments was enhanced using WPM (woody plant medium) medium lacking growth regulators. From various shoot multiplication treatments, the highest number of shoots per explant (30.4) was found on ME (Media created specifically) medium supplemented with 0.8mgl−1 BAP and 0.05mgl−1 IBA. 100% in vitro rooting was achieved on ½ strength MS medium with 0.5mgl−1 IBA, 1.6mgl−1 thiamine and 150mgl−1 iron sequestrene.
ISSN:1687-157X
2090-5920
DOI:10.1016/j.jgeb.2014.12.006