Membrane Technology in the Production of Immunobiological Preparations Produced by the Branch Office of the FSBE «48 Central Scientific Research Institute» of the Ministry of Defence of Russia (Kirov)

The researchers of the Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation (Kirov) organized theoretical and experimental studies on the introduction of a tangential filtration method for the separ...

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Veröffentlicht in:Вестник войск РХБ защиты 2023-07, Vol.3 (2), p.137-149
1. Verfasser: , Krupin V.V, Leshchenko A.A.
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Sprache:rus
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Zusammenfassung:The researchers of the Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation (Kirov) organized theoretical and experimental studies on the introduction of a tangential filtration method for the separation of biological mixtures into the production of immunobiological preparations. The method of microfiltration in the tangential stream replaced the process of sedimentation at the stage of concoction of intermediate vaccines, reduced considerably the process time, and allowed to obtain suspensions from cultural liquid, substandard on an indicator of concentration of microbial cells. Along with this, the microfiltration method allowed to concentrate the cultures of Yersinia pestis of a vaccinal strain EV. In comparison with the centrifugal separation, the concentration of living microbial cells of a vaccinal strain of EV Y. pestis increased by one and a half times. The filtration in a tangential stream at the ASF-020 installation from the point of view of the production of the sporous product of anthrax vaccine STI-1 (in millions of doses), is 1.8 times more effective in comparison with the centrifugal separation. The membrane method allowed to reduce the duration of technological process. These membrane processes are used nowadays during the production of plague and anthrax vaccines, anthrax immunoglobulin, diagnostic medicines and during the sterilization of liquid nutrient mediums. This type of equipment for the sterilization of nutrient mediums can be considered as an alternative to the processes of the thermal sterilization of liquids and provides their biological and technological full functionality. Experiments on the use of the ceramic-metal filters sterilizing the air given for aeration, showed the decrease in duration of preparatory operations on 20 h and increase in the general operational opportunities of the system.
ISSN:2587-5728
DOI:10.35825/2587-5728-2019-3-2-137-149