Bacterial pore-forming toxin pneumolysin drives pathogenicity through host extracellular vesicles released during infection

Streptococcus pneumoniae is a global priority respiratory pathogen that kills over a million people annually. The pore-forming cytotoxin, pneumolysin (PLY) is a major virulence factor. Here, we found that recombinant PLY as well as wild-type pneumococcal strains, but not the isogenic PLY mutant, upregulated the shedding of extracellular vesicles (EVs) harboring membrane-bound toxin from human THP-1 monocytes. PLY-EVs induced cytotoxicity and hemolysis dose-dependently upon internalization by recipient monocyte-derived dendritic cells. Proteomics analysis revealed that PLY-EVs are selectively enriched in key inflammatory host proteins such as IFI16, NLRC4, PTX3, and MMP9. EVs shed from PLY-challenged or infected cells induced dendritic cell maturation and primed them to infection. In vivo, zebrafish administered with PLY-EVs showed pericardial edema and mortality. Adoptive transfer of bronchoalveolar-lavage-derived EVs from infected mice to healthy recipients induced lung damage and inflammation in a PLY-dependent manner. Our findings identify that host EVs released during infection mediate pneumococcal pathogenesis. [Display omitted] •Bacterial cytotoxin, pneumolysin (PLY), upregulates shedding of host vesicles•Toxin transfer to recipient cells upon vesicle internalization induces cytotoxicity•PLY-induced vesicles are enriched for antimicrobial and inflammatory host proteins•Transfer of vesicles isolated from infected to healthy mice induces pathogenicity Microbiology; Bacteriology; Cell biology