Qualitative TLC determination of some polycyclic aromatic hydrocarbons in sugar-beet

The presence of polycyclic or polynuclear aromatic hydrocarbons (PAHs) were investigated in sugar-beet from a local sugar factory in the district of Vojvodina. The sugar-beet was cultivated on areas near roads with intensive traffic. The procedure for the preparation and determination of these compounds included saponification of the sample, several liquid?liquid extraction systems and a silica gel column clean-up. The purified sample solution was analysed by thin layer chromatography (TLC) on silica gel with cyclohexane as the developing solvent. Benzo(b)fluoranthene and benzo(a)anthracene and/or benzo(a)pyrene were detected at concentrations greater than the allowed limits in food. Ispitivano je prisustvo policiklicnih aromaticnih ugljovodonika u secernoj repi iz lokalne secerane na podrucju AP Vojvodine. Secerna repa je gajena na povrsinama blizu puteva sa intenzivnim saobracajem. Postupak za pripremu i odredjivanje ovih jedinjenja ukljucuje saponifikaciju uzorka, nekoliko ekstrakcija tecno?tecno i preciscavanje na koloni silika gela. Preciscen uzorak je analiziran hromatografijom na tankom sloju silika gela sa cikloheksanom kao mobilnom fazom. U uzorku secerne repe je utvrdjeno prisustvo benzo(b)fluorantena i benzo(a)antracena i/ili benzo(a)pirena iznad granica detekcije u hrani.