Developmental Roles of FUSE Binding Protein 1 ( Fubp1 ) in Tooth Morphogenesis
FUSE binding protein 1 ( ), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of , we examined the detailed expression pattern...
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Veröffentlicht in: | International journal of molecular sciences 2020-10, Vol.21 (21), p.8079 |
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Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | FUSE binding protein 1 (
), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of
, we examined the detailed expression pattern and developmental function of
during tooth morphogenesis by RT-qPCR, in situ hybridization, and knock-down study using in vitro organ cultivation methods. In embryogenesis,
is obviously expressed in the enamel organ and condensed mesenchyme, known to be important for proper tooth formation. Knocking down
at E14 for two days, showed the altered expression patterns of tooth development related signalling molecules, including
and
. In addition, transient knock-down of
at E14 revealed changes in the localization patterns of c-Myc and cell proliferation in epithelium and mesenchyme, related with altered tooth morphogenesis. These results also showed the decreased amelogenin and dentin sialophosphoprotein expressions and disrupted enamel rod and interrod formation in one- and three-week renal transplanted teeth respectively. Thus, our results suggested that
plays a modulating role during dentinogenesis and amelogenesis by regulating the expression pattern of signalling molecules to achieve the proper structural formation of hard tissue matrices and crown morphogenesis in mice molar development. |
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ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms21218079 |