Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful...

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Veröffentlicht in:	Stem cell research 2021-04, Vol.52, p.102238-102238, Article 102238
Hauptverfasser:	Sims, C.H. Cole, Autio, Matias I., Buskin, Adriana, Cheung, Christine, Heer, Rakesh, Foo, Roger S.Y., Wang, Xin
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Cas Systems - genetics Gene Knockout Techniques Human Embryonic Stem Cells Humans
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2021.102238