Basidiospores from Wood-Decay Fungi Transform Laccase Substrates in the Absence of Glucose and Nitrogen Supplements

Preparations of bacterial endospores and fungal conidia are applied in biocontrols, biocatalyses, and lignocellulose fermentations. The biocatalytic abilities of basidiospores from mushrooms of the order Agaricales are unknown. To assess their potential in colonizing recalcitrant substrates solely with their inherent resources, spores of the white-rot fungi (Stru) and (Kmt, Strophariaceae) were analyzed for surface-bound and internal total carbohydrates, phenols, proteins, minerals, and oxidoreductases to estimate their chemistry and the preconditions to transform the laccase substrates guaiacol and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) independent of external glucose and nitrogen. Surfaces of Stru/Kmt spores released (mg kg ) hexoses, 7300/9700; phenols, >62/220; proteins, 21/168; and laccases, 42/0-0.15 µmol ABTS kg min that mimicked oxidative activities of the resting spores. Milled-spore extracts contained pentoses, 96,600/6750; hexoses, 160,000/15,130; phenols, 452/767; protein, 12,600/924; true laccase, 688/0.30; and enzyme-protein-activating transition metals such as Cu in concentrations typical of wheat grains. Independent of external N and C supply, spores (