A simple electrical stimulation cell culture system on the myelination of dorsal root ganglia and Schwann cells

A large number of animal experiments and clinical trials have confirmed that electrical stimulation can accelerate the growth of axons and recovery of motor function, all of which are inseparable from the formation of myelin. Therefore, establishment of a suitable electrical stimulation platform to study the effects of electrical stimulation on the myelin process of dorsal root ganglia and Schwann cells is of great significance for understanding the recovery of electrical stimulation. We designed a simple conductive glass cell culture system to overcome the shortcomings of direct contact of the electrode with the culture solution, and the number of culture chambers can be selected based on the purpose of the experiment in order to reduce experimental time and cost. Here, we propose a new electrical stimulation cell culture system for exposing neurons/Schwann cells to electrical stimulation to induce cell differentiation, migration and myelination. The device is simple, convenient and can be used to study the electrical stimulation effect and electrophysiological characteristics of nerve cells .