Development of a UPLC-MS/MS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats

Development of a UPLC-MS/MS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats

VS-5584 is a small-molecular compound that showed equivalent activity against mTOR and all class I PI3K isoforms and demonstrated preclinical activity in diverse cancer cell lines and xenograft tumor model, and rational combination of VS-5584 and other target therapies achieved promising outcomes in...

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Veröffentlicht in:Journal of analytical methods in chemistry 2020, Vol.2020 (2020), p.1-6
Hauptverfasser: Fang, Lina, Tian, Jinmiao, Liu, Tianzhu, He, Aiping, Li, Xiaoxin, Zhou, Chunling, Jiang, Meihua
Format: Artikel
Sprache:eng
Schlagworte:
Accuracy
Acetonitrile
Acids
Apoptosis
Calibration
Cancer
Chromatography
Formic acid
Linearity
Melanoma
Methods
Oral administration
Organic acids
Pharmacokinetics
Pharmacology
Plasma
Quality control
Xenotransplantation
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Zusammenfassung:VS-5584 is a small-molecular compound that showed equivalent activity against mTOR and all class I PI3K isoforms and demonstrated preclinical activity in diverse cancer cell lines and xenograft tumor model, and rational combination of VS-5584 and other target therapies achieved promising outcomes in oncology. In the present study, we established and validated a simple and sensitive UPLC-MS/MS method for the determination of VS-5584 in plasma samples. VS-5584 was separated via an Acquity UPLC BEH C18 column, with a mobile phase composed of acetonitrile and 0.2% formic acid in water (40 : 60). The calibration curve displayed a good linearity in the range of 1.0–1000 ng/mL, with satisfactory accuracy (−13.6% 
ISSN:2090-8865
2090-8873
DOI:10.1155/2020/8811522