Evaluation of the Protective Immune Response Induced by an rfbG- Deficient Salmonella enterica Serovar Enteritidis Strain as a Live Attenuated DIVA (Differentiation of Infected and Vaccinated Animals) Vaccine in Chickens

Salmonella enterica serovar Enteritidis ( . Enteritidis), one of the zoonotic pathogens, not only results in significant financial losses for the global poultry industry but also has the potential to spread to humans through poultry and poultry products. Vaccination is an effective method to prevent infections. In this study, we constructed a live attenuated DIVA (differentiation of infected and vaccinated animals) vaccine candidate, Z11Δ , and evaluated its protective effectiveness and DIVA potential in chickens. Compared to that of the virulent wild-type strain, the 50% lethal dose (LD ) of the mutant strain increased 56-fold, confirming its attenuation. High serum levels of . Enteritidis-specific IgG titers indicated that a significant humoral immune response was induced in the vaccinated group. After challenge, the nonvaccinated group showed serious clinical symptoms (diarrhea, depression, decreased appetite, ruffled feathers, and weight loss), pathological changes (white nodules in the liver and fatty lesions in liver cells), and death. In contrast, there were no clinical symptoms, pathological changes, or death in the 5 × 10 - and 5 × 10 -CFU-vaccinated groups. Z11Δ vaccination significantly reduced . Enteritidis colonization in the spleen, liver, and cecum. In addition, the Z11Δ -vaccinated group exhibited a negative response to the serological test, whereas the virulent wild-type Z11 infection group was strongly positive for the serological test, showing a DIVA capability of Z11Δ vaccination. Overall, our findings demonstrate the viability of the mutant as a live attenuated chicken vaccine that can discriminate between animals that have been immunized and those that have been infected. . Enteritidis is a highly adapted pathogen that causes significant economic losses in the poultry industry around the world. Vaccination is an effective method of controlling . Enteritidis infections. Here, we demonstrated that . Enteritidis Z11Δ has the potential to be a safe, immunogenic, and DIVA vaccine candidate for the control of infections in chickens. Z11Δ not only provided effective protection in chickens but also distinguished between infected and vaccinated chickens by serological tests.