Picornavirus translation strategies

The genome of viruses classified as picornaviruses consists of a single monocistronic positive strand RNA. The coding capacity of these RNA viruses is rather limited, and thus, they rely on the cellular machinery for their viral replication cycle. Upon the entry of the virus into susceptible cells, the viral RNA initially competes with cellular mRNAs for access to the protein synthesis machinery. Not surprisingly, picornaviruses have evolved specialized strategies that successfully allow the expression of viral gene products, which we outline in this review. The main feature of all picornavirus genomes is the presence of a heavily structured RNA element on the 5´UTR, referred to as an internal ribosome entry site (IRES) element, which directs viral protein synthesis as well and, consequently, triggers the subsequent steps required for viral replication. Here, we will summarize recent studies showing that picornavirus IRES elements consist of a modular structure, providing sites of interaction for ribosome subunits, eIFs, and a selective group of RNA‐binding proteins. Upon the entry of the viral RNA into susceptible cells, cis‐ and trans‐regulatory elements coordinately determine picornavirus protein synthesis. Early in the infection, the cap‐dependent translation of host mRNAs is shut down, while viral RNA takes advantage of the IRES element, resulting in efficient synthesis of viral proteins. In this review, we discuss RNA structural motifs and host factors influencing viral RNA translation.