Study of Essential Oil and Antioxidant Capacity of Clematis ispahanica Boiss

Clematis ispahanica Boiss. is a rare species belonging to the Ranunculaceae family. Clematis ispahanica is highly valued for its ornamental and medicinal properties. The specific traditional uses of Clematis ispahanica in traditional medicine may include curing neurological disorders, syphilis, malaria, rheumatism, gout, diarrhea, and asthma. In this study, leaves of C. ispahanica were collected from collected from Boanat, Kerman Province, Iran. Essential oil isolated from the leaves of C. ispahanica was evaluated for its chemical composition for the first time. GC/MS analyzed the essential oil obtained by hydrodistillation. The chemical analysis of the oil from Clematis ispahanica Boiss. revealed the identification of 50 compounds, accounting for 92.5% of the total oil composition. In the leaves oil, the main components detected were phthalic acid, heptacosane, pentadecanoic acid, methyl ester, and a-pinene with amounts of 51.1 %, 5.8 %, 3.9 %, and 2.9 %, respectively. The analysis of various extracts from Clematis ispahanica Boiss. revealed a significant difference among all of them in Antioxidant activity (AA), Total phenol content (TPC) and Total flavonoid content (TFC) (p≤0.01, p≤0.05). For the antioxidant activity, the results obtained showed that the highest radical scavenging activity was observed in the extract of hexane with IC50 122 μg/ml, and the lowest activity was found in acetone extraction with IC50 170 μg/ml. The highest TPC value was 15.0 mg GAE/g DW in the extract of hexane. The highest TFC belongs to the extract of hexane and methanol with 7.3 mg QE/g and 7.2 mg QE/g. The analysis between TPC (r= 0.708, p≤0.01) and TFC (r= 0.786, p≤0.01) of C. ispahanica and its antioxidant properties were found to have a strong correlation. C. ispahanica exhibits a relatively high level of antioxidant potency and contains a significant amount of total phenolic compounds when extracted with a hexane solvent.