3D TECA hydrogel reduces cellular senescence and enhances fibroblasts migration in wound healing

Three dimensional hydrogel containing titrated extract of Centella asiatica was prepared following Trojan et al. (2005) with slight medication. The hydrogel was cultured with periodontal ligament fibroblasts which are triggered with TNF-α, then the senescence marker of fibroblasts was measured. Migration of fibroblasts using in vitro wound healing assay was also evaluated. [Display omitted] This study was designed to investigate the effect of 3D TECA hydrogel on the inflammatory-induced senescence marker, and to assess the influence of the gel on the periodontal ligament fibroblasts (PDLFs) migration in wound healing in vitro. PDLFs were cultured with 20 ng/ml TNF-α to induce inflammation in the presence and absence of 50 µM 3D TECA gel for 14 d. The gel effect on the senescence maker secretory associated-β-galactosidase (SA-β-gal) activity was measured by a histochemical staining. Chromatin condensation and DNA synthesis of the cells were assessed by 4′,6-diamidino-2-phenylindole and 5-ethynyl-2′-deoxyuridine fluorescent staining respectively. For evaluating fibroblasts migration, scratch wound healing assay and Pro-Plus Imaging software were used. The activity of senescence marker, SA-β-gal, was positive in the samples with TNF-α-induced inflammation. SA-β-gal percentage is suppressed (>65%, P