Environmental DNA‐derived pathogen gene sequences can expand surveillance when pathogen titers are decoupled in eDNA and hosts

Environmental DNA (eDNA) collection has emerged as a powerful and noninvasive wildlife population and pathogen‐monitoring tool. Ranavirus is an emerging pathogen linked to die‐offs in amphibian species. Applications of eDNA detection for ranavirus surveillance have shown promise, but it is unclear h...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Environmental DNA 2021-11, Vol.3 (6), p.1192-1207
Hauptverfasser:	Kaganer, Alyssa W., Nagel, Leah D., Youker‐Smith, Tess E., Bunting, Elizabeth M., Hare, Matthew P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amphibians biological monitoring Catchment basins communicable diseases Deoxyribonucleic acid DNA emerging Epidemics Frogs Gene sequencing Genes Genetic polymorphisms Health aspects Infection Infections Larvae Liver nonlethal sampling Nucleotide sequence Pathogens ponds Populations Ranavirus Reptiles & amphibians Seasons Viruses Wildlife
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Environmental DNA (eDNA) collection has emerged as a powerful and noninvasive wildlife population and pathogen‐monitoring tool. Ranavirus is an emerging pathogen linked to die‐offs in amphibian species. Applications of eDNA detection for ranavirus surveillance have shown promise, but it is unclear how reliably eDNA represents viral pressure on host populations. We evaluated the relationship of ranavirus titers in hosts and eDNA in four New York vernal pools from May to October 2016. We compared ranavirus titers in aquatic eDNA samples (n = 562) and liver samples (n = 362) of wood frog (Lithobates sylvaticus) and green frog (Lithobates clamitans) larvae from pools with past ranavirus die‐offs. We detected low‐quantity Ranavirus DNA (
ISSN:	2637-4943 2637-4943
DOI:	10.1002/edn3.240