Differential gene expression (DGE) analysis in persons with a history of giardiasis
Giardiasis, which is caused by Giardia duodenalis , has clinical symptoms such as steatorrhea and can be very dangerous in children . In addition, some documents reported that this parasite is present inside the tissue of patients with cancer. In this study, we analyzed the gene expression profiles...
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Veröffentlicht in: | AMB Express 2024-01, Vol.14 (1), p.1-1, Article 1 |
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Zusammenfassung: | Giardiasis, which is caused by
Giardia duodenalis
, has clinical symptoms such as steatorrhea and can be very dangerous in children
.
In addition, some documents reported that this parasite is present inside the tissue of patients with cancer. In this study, we analyzed the gene expression profiles of some main genes important to apoptosis and anti-apoptosis in humans.
Expression profile arrays of Genomic Spatial Event (GSE) 113666, GSE113667, and GSE113679 obtained from Gene Expression Omnibus were used for meta-analysis using R commands. Cytoscape and STRING databases used the protein–protein Interaction network. Then, the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis was performed. Similar genes in
Homo sapiens
were identified using Basic Local Alignment Search Tool analysis. The validation was performed on eight people using real-time Polymerase chain reaction. In addition to the candidate genes, the gene expression of some other genes, including
Serine/Threonine Kinase
1 (
AKT1
),
Cyclin Dependent Kinase Inhibitor 2A
(
CDKN2A
),
Kirsten Rat Sarcoma
(
KRAS
), and
Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha
(
PIK3CA
) were also examined. Analysis of the expression of
serum amyloid A1
(
SAA1
)
,
Regenerating Islet-Derived 3 Gamma
(
REG3G
)
,
and
REG3A
genes did not show any difference between the two groups of healthy and diseased people. Examining the mean expression of the four genes
AKT1, CDKN2A, KRAS,
and
PIK3CA
showed that three genes of
AKT1, CDKN2A,
and
KRAS
had increased expression in people with a history of giardiasis compared to healthy people. We showed that the gene expression pattern differs in apoptosis and anti-apoptosis signaling in people with a history of giardiasis.
Giardia duodenalis
seems to induce post-non-infectious symptoms with stimulation of human gene expression. |
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ISSN: | 2191-0855 2191-0855 |
DOI: | 10.1186/s13568-023-01657-1 |