Reproductive toxicity of aqueous wood-ash extract of Azadirachta indica (neem) on male albino mice

To evaluate the reproductive toxicity of aqueous wood ash extract of Azadirachta indica (A. indica) in male albino mice. Four different dose levels of 0, 5, 50 and 100 mg/kg body weight were administered to 20 male mice, with five mice per group for seven days that were sacrificed 35 days thereafter. Gonadosomatic index, sperm motility, sperm count, sperm morphology, serum follicle stimulating hormone (FSH), leuitinizing hormone (LH) and testosterone assay, and histopathology of testes were carried out. Though no toxic effect on testicular weight, FSH, LH and testosterone (P > 0.05), significant decrease in sperm motility, live/dead sperm and sperm count, with significant increase of abnormal sperm were recorded (P