Developmental-stage specific single-cell human embryo dissociation

Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing e...

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Veröffentlicht in:STAR protocols 2023-09, Vol.4 (3), p.102363-102363, Article 102363
Hauptverfasser: Lammers, Jenna, Loubersac, Sophie, David, Laurent, Freour, Thomas, Reignier, Arnaud
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Sprache:eng
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Zusammenfassung:Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, and dispense cells in plates. [Display omitted] •Technique to isolate embryo cells by mechanical dissociation•Technique suitable from cell stage to blastocyst Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, and dispense cells in plates.
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2023.102363