Tanshinone ⅡA attenuates high glucose-induced apoptosis and oxidative stress in mouse podocyte line PMC-5

Objective To explore potential mechanisms involved in the reduction of podocyte apoptosis and oxidative stress induced by high glucose (HG) by tanshinone ⅡA (Tan ⅡA). Methods CCK-8 method was used to screen the appropriate interference concentration of Tan ⅡA. The MPC-5 cells were divided into NC group (5.5 mmol/L D-glucose), MA group(5.5 mmol/L D-glucose and 22.5 mmol/L mannitol), HG group(25 mmol/L D-glucose), HG+Tan ⅡA group(25 mmol/L D-glucose and 10 μmol/L Tan ⅡA), and HG+Tan ⅡA+SIRT1 inhibitors(sirtinol) group (25 mmol/L D-glucose, 10 μmol/L Tan ⅡA and 20 μmol/L sirtinol). Determina- tion of oxidative stress markers malondialdehyde(MDA), superoxide dismutase (SOD) and catalase (CAT) were made by microplate method, WST-1 method and visible light method. Flow cytometry was used to measure cell apoptosis. Western blot was used to evaluate the expression of apoptosis-related proteins and silent information regulator 1 (SIRT1)/endothelial nitric oxide synthase (eNOS) pathway proteins. Results Tan ⅡA improved