In situ Impact of the Antagonistic Fungal Strain, Trichoderma gamsii T30 on the Plant Pathogenic Fungus, Rhizoctonia solani in Soil

is a soil-borne fungus causing a wide range of plants diseases. strain T30 has previously been reported as antagonistic against . Although there are a few studies about the influence of strains on the density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes effects of a strain on the population density of in the soil microcosmic conditions. The population dynamics of were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of / ). The population density of was evaluated by qPCR. In the autoclaved soil, target DNA copies of increased in the control samples from 1 × 10 to 6.5 × 10 . At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 10 and 7.6 × 10 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal cells decreased at day 25 whether inoculated or not with indicating a general suppression by the soil microbiome. In brief, significantly inhibited the growth of in the soil and there was a general suppressive effect of the natural microbiome. is a soil-borne fungus causing a wide range of plants diseases. strain T30 has previously been reported as antagonistic against . Although there are a few studies about the influence of strains on the density in a pathosystem in the presence of plant hosts, this report for the first time comprehensively describes effects of a strain on the population density of in the soil microcosmic conditions. The population dynamics of were followed in the autoclaved and non-autoclaved soils in artificially prepared microcosms up to day 25 after co-inoculation with in the variable ratios (R1/T1; R1/T0.1; R1/T0.01 of / ). The population density of was evaluated by qPCR. In the autoclaved soil, target DNA copies of increased in the control samples from 1 × 10 to 6.5 × 10 . At R1/T0.01, the number of target DNA copies were not significantly changed until day 11; however, it decreased by around five times at day 25. At R1/T0.1 and R1/T1, the number of DNA copies was reduced to 2.1 × 10 and 7.6 × 10 at day 11, respectively and the reduction was as much as 17 times at day 25. In the non-autoclaved soil, the number of the fungal ce