Bioinformatic prediction and functional characterization of human KIAA0100 gene

Our previous study demonstrated that human KIAA0100 gene is a novel acute monocytic leukemia-associatedantigen (MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown todate. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using online software;Secondly, human KIAA0100 gene expression was downregulated by the clustered regularly interspaced shortpalindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system in U937 cells. Cell proliferation andapoptosis were next evaluated in KIAA0100-knockdown U937 cells. The bioinformatic prediction showed thathuman KIAA0100 gene was located on 17q11.2, and human KIAA0100 protein was located in the secretorypathway. Besides, human KIAA0100 protein contained a signal peptide, a transmembrane region, three types ofsecondary structures (alpha helix, extended strand, and random coil) , and four domains from mitochondrialprotein 27 (FMP27). The observation on functional characterization of human KIAA0100 gene revealed that itsdownregulation inhibited cell proliferation, and promoted cell apoptosis in U937 cells. To summarize, theseresults suggest human KIAA0100 gene possibly comes within mitochondrial genome; moreover, it is a novelanti-apoptotic factor related to carcinogenesis or progression in acute monocytic leukemia, and may be apotential target for immunotherapy against acute monocytic leukemia.