Cell Growth Stimulation, Cell Cycle Alternation, and Anti-Apoptosis Effects of Bovine Bone Collagen Hydrolysates Derived Peptides on MC3T3-E1 Cells Ex Vivo

Bovine bone collagen hydrolysates promote bone formation through regulating bone growth. However, the peptide sequences within these isolates have not been characterized. In this study, twenty-nine peptides from bovine bone collagen hydrolysates were purified and identified using nano-HPLC-MS-MS and...

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Veröffentlicht in:	Molecules (Basel, Switzerland) Switzerland), 2020-05, Vol.25 (10), p.2305
Hauptverfasser:	Wang, Jianing, Liu, Junli, Guo, Yanchuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Amino Acid Sequence Animals Antioxidants Apoptosis Apoptosis - drug effects Binding Sites Biological activity Biomedical materials Bone and Bones - chemistry Bone growth Bones bovine bonecollagen hydrolysates Cattle Cell cycle Cell Cycle - drug effects cell cycle distribution Cell Differentiation - drug effects Cell Division - drug effects Cell growth Cell Line Cell Survival - drug effects Cell viability Collagen Collagen - chemistry Collagen - isolation & purification EGFR Epidermal growth factor Epidermal growth factor receptors ErbB Receptors - chemistry ErbB Receptors - genetics ErbB Receptors - metabolism Growth factors High-performance liquid chromatography Hydrogen Bonding Hydrogen bonds Hydrolysates Hydrophobic and Hydrophilic Interactions Hydrophobicity Ligands Liquid chromatography Mass spectrometry MC3T3-E1 cells Mice Molecular docking Molecular Docking Simulation Molecular weight Osteoblasts - cytology Osteoblasts - drug effects Osteogenesis Osteoporosis Peptides Peptides - chemistry Peptides - genetics Peptides - metabolism Peptides - pharmacology proliferation Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Hydrolysates - chemistry Protein Interaction Domains and Motifs Scientific imaging Signal transduction Skin Vertebrae
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Zusammenfassung:	Bovine bone collagen hydrolysates promote bone formation through regulating bone growth. However, the peptide sequences within these isolates have not been characterized. In this study, twenty-nine peptides from bovine bone collagen hydrolysates were purified and identified using nano-HPLC-MS-MS and Peak Studio analysis. HHGDQGAPGAVGPAGPRGPAGPSGPAGKDGR (Deamidation) and GPAGANGDRGEAGPAGPAGPAGPR (Deamidation) enhanced cell viability, inhibited apoptosis, and significantly altered the cell cycle of MC3T3-E1 osteoblast cells. These peptides were selected to perform molecular docking analysis to examine the mechanism underlying these bioactivities. Molecular docking analysis showed that these two peptides formed hydrophobic interactions and hydrogen bonds with epidermal growth factor receptor (EGFR) to activate the EGFR-signaling pathway, which may explain their bioactivity. These findings indicate that these and other similar peptides might be candidates for the treatment of osteoporosis.
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules25102305