Prolonged DNA hydrolysis in water: A study on DNA stability

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are desc...

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Veröffentlicht in:	Data in brief 2018-10, Vol.20, p.1237-1243
Hauptverfasser:	Fattorini, Paolo, Marrubini, Giorgio, Bonin, Serena, Bertoglio, Barbara, Grignani, Pierangela, Recchia, Elisa, Pitacco, Paola, Procopio, Francesca, Cantoni, Carolina, Pajnič, Irena Zupanič, Sorçaburu-Cigliero, Solange, Previdere`, Carlo
Format:	Artikel
Sprache:	eng
Schlagworte:	Degraded samples DNA hydrolysis DNA quantification Physics and Astronomy qPCR
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study.
ISSN:	2352-3409 2352-3409
DOI:	10.1016/j.dib.2018.08.120