Antimicrobial Resistance Profile of mcr-1 Positive Clinical Isolates of Escherichia coli in China From 2013 to 2016

Multidrug-resistant (MDR) poses a great challenge for public health in recent decades. Polymyxins have been reconsidered as a valuable therapeutic option for the treatment of infections caused by MDR A plasmid-encoded colistin resistance gene encoding phosphoethanolamine transferase has been recently described in Enterobacteriaceae. In this study, a total of 123 . isolates obtained from patients with diarrheal diseases in China were used for the genetic analysis of colistin resistance in clinical isolates. Antimicrobial resistance profile of polymyxin B (PB) and 11 commonly used antimicrobial agents were determined. Among the 123 isolates, 9 isolates (7.3%) were resistant to PB and PCR screening showed that seven (5.7%) isolates carried the gene. A hybrid sequencing analysis using single-molecule, real-time (SMRT) sequencing and Illumina sequencing was then performed to resolve the genomes of the seven positive isolates. These seven isolates harbored multiple plasmids and are MDR, with six isolates carrying one positive plasmid and one isolate (14EC033) carrying two positive plasmids. These eight positive plasmids belonged to the IncX4, IncI2, and IncP1 types. In addition, the gene was the solo antibiotic resistance gene identified in the positive plasmids, while the rest of the antibiotic resistance genes were mostly clustered into one or two plasmids. Interestingly, one positive isolate (14EC047) was susceptible to PB, and we showed that the activity of MCR-1-mediated colistin resistance was not phenotypically expressed in 14EC047 host strain. Furthermore, three isolates exhibited resistance to PB but did not carry previously reported -related genes. Multilocus sequence typing (MLST) showed that these positive . isolates belonged to five different STs, and three isolates belonged to ST301 which carried multiple virulence factors related to diarrhea. Additionally, the positive isolates were all susceptible to imipenem (IMP), suggesting that IMP could be used to treat infection caused by positive isolates. Collectively, this study showed a high occurrence of positive plasmids in patients with diarrheal diseases of Guangzhou in China and the abolishment of the MCR-1 mediated colistin resistance in one isolate.