Molecular Detection and Characterisation of Coxiella burnetii in Koala ( Phascolarctos cinereus ) Urogenital Tract Swabs

Q fever is a zoonosis caused by , primarily affecting those in close contact with domestic ruminants, the main source of human infection. has also been detected in various wildlife species globally. In Australia, serological and molecular studies have shown exposure to and infection by in macropods, bandicoots, and koalas. However, the extent to which these species contribute to human infection remains unclear. An unpublished public health investigation into a Q fever case in a person involved in koala care could not conclusively link the infection to koalas due to the patient's broad animal exposure. This study aimed to explore the potential role of koalas in transmitting to humans by investigating the presence of DNA in urogenital tract (UGT) swabs from koalas. DNA was extracted from UGT swabs from koalas in three regions in New South Wales, Australia. An optimised multiplex qPCR assay detected DNA in 2 out of 225 samples (0.89%) at approximately 10 genome equivalents per reaction. Both positive samples amplified all three gene targets. MLVA genotyping identified two distinct genotypes previously isolated from Australian Q fever cases. These findings highlight the need for vaccination against Q fever for those in close contact with koalas.