Antibacterial Activity of Endophytic Actinomycetes Isolated from the Medicinal Plant Vochysia divergens (Pantanal, Brazil)

Endophytic actinomycetes from medicinal plants produce a wide diversity of secondary metabolites (SM). However, to date, the knowledge about endophytes from Brazil remains scarce. Thus, we analyzed the antimicrobial potential of 10 actinomycetes isolated from the medicinal plant located in the Pantanal sul-mato-grossense, an unexplored wetland in Brazil. Strains were classified as belonging to the , and genera, through morphological and 16S rRNA phylogenetic analyzes. A susceptibility analysis demonstrated that the strains were largely resistant to the antibiotics oxacillin and nalidixic acid. Additionally, different culture media (SG and R5A), and temperatures (28 and 36°C) were evaluated to select the best culture conditions to produce the active SM. All conditions were analyzed for active metabolites, and the best antibacterial activity was observed from metabolites produced with SG medium at 36°C. The LGMB491 (close related to ) extract showed the highest activity against methicillin-resistant (MRSA), with a MIC of 0.04 mg/mL, and it was selected for SM identification. Strain LGMB491 produced 1-acetyl-β-carboline ( ), indole-3-carbaldehyde ( ), 3-(hydroxyacetyl)-indole ( ), brevianamide F ( ), and cyclo-(L-Pro-L-Phe) ( ) as major compounds with antibacterial activity. In this study, we add to the knowledge about the endophytic community from the medicinal plant and report the isolation of rare actinomycetes that produce highly active metabolites.