Regulatory Effects of miR-205 on Invasion and Migration of Gastric Cancer Cells and Potential Mechanism

Regulatory Effects of miR-205 on Invasion and Migration of Gastric Cancer Cells and Potential Mechanism

Objective To investigate the effect of miR-205 on the invasion and migration of HGC27 cells and related mechanism. Methods The expression of miR-205 in four kinds of gastric cancer cell lines (AGS, MKN74, HGC27, SGC7901) were measured by real-time quantitative PCR. miR-205 mimic was transfected into...

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Veröffentlicht in:Zhongliu fangzhi yanjiu 2018-05, Vol.45 (5), p.280-284
Hauptverfasser: Wang, Yuxia, Wang, Fang, Cui, Huasheng, Wang, Huimin, Shen, Feifei, Suo, Shenghong
Format: Artikel
Sprache:chi
Schlagworte:
Cancer
Cell migration
E-cadherin
emt
Gastric cancer
invasion
Mesenchyme
migration
mir-205
N-Cadherin
Proteins
Real time
Scratch tests
Transfection
Tumor cell lines
Vimentin
β-Catenin
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Zusammenfassung:Objective To investigate the effect of miR-205 on the invasion and migration of HGC27 cells and related mechanism. Methods The expression of miR-205 in four kinds of gastric cancer cell lines (AGS, MKN74, HGC27, SGC7901) were measured by real-time quantitative PCR. miR-205 mimic was transfected into HGC27 cells for up-regulation of miR-205, then cells invasion and migration were detected by scratch test and Transwell assay. Furthermore, epithelial to mesenchymal transition (EMT) progress and the activation of related signal pathway were measured by Western blot. Results Real-time quantitative PCR showed that miR-205 was significantly down-regulated in gastric cancer cells (all P
ISSN:1000-8578
1000-8578
DOI:10.3971/j.issn.1000-8578.2018.17.1217