Insights into the role of hexa-bacterial consortium for bioremediation of soil contaminated with chlorantraniliprole
This study evaluates the efficacy of discrete bacterial consortia in bioremediating sandy loamy soil deliberately contaminated with 20 mg/kg of chlorantraniliprole (CAP). It monitors alterations in total bacterial populations and CO 2 emissions, tracking residual CAP levels through UV scanning and H...
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Veröffentlicht in: | Environmental sciences Europe 2024-11, Vol.36 (1), p.197-16, Article 197 |
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Zusammenfassung: | This study evaluates the efficacy of discrete bacterial consortia in bioremediating sandy loamy soil deliberately contaminated with 20 mg/kg of chlorantraniliprole (CAP). It monitors alterations in total bacterial populations and CO
2
emissions, tracking residual CAP levels through UV scanning and HPLC analysis. Six active bacterial degraders (four
Bacillus
strains (
B. subtilis
subsp
. subtilis
AZFS3
, B. pumilus
AZFS5
, B. mojavensis
AZFS15, and
B. paramycoides
AZFS18), one
Alcaligenes
strain (
A. aquatilis
KZFS11), and one
Pseudomonas
strain (
P. aeruginosa
KZFS4)) were used in single or combined preparations and grown on trypticase soy broth for 24 h at 30 °C before preparing the inoculants and adjusting the bacterial cell count to 10
7
CFU/dwt g soil. The bacterial consortia were added to the CAP-contaminated soil and incubated for 20 days at 30 °C. The di-, tetra-, and hexa-bacterial consortia recorded the highest levels of viable bacteria, reaching their peak after 3 to 11 days of incubation. Then, they declined to the minimum levels at the end of the 20 days, which coincided with their complete removal of CAP from the soil. At the end of the incubation period (20 days), the CAP was mainly biodegraded, scoring biodegradation rates of 90.05%, 93.65%, and 98.65% for T3, T4, and T5, respectively. This concurred with the highest average CO
2
production. Based on the results of the HPLC analysis, the hexa-bacterial consortium T5 demonstrated the highest rate of CAP biodegradation (99.33%) after a 20-day incubation period, resulting in the lowest residual level of CAP in the soil (0.67%). Bioinformatic analysis predicted that the CAP biodegradation pathway reached CO
2
and H
2
O. Under optimized conditions, the hexa-bacteria consortium is the most effective CAP biodegraded and is recommended as an eco-friendly treatment for eliminating CAP pollution in the field. |
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ISSN: | 2190-4715 2190-4715 |
DOI: | 10.1186/s12302-024-01008-2 |