Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a n...

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Veröffentlicht in:	Sensors (Basel, Switzerland) Switzerland), 2018-12, Vol.19 (1), p.52
Hauptverfasser:	Jeong, Hee-Jin, Dong, Jinhua, Ueda, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Anti-Idiotypic - immunology Antigens Binding sites Communication Epidemics Fluorescence H1N1 hemagglutinin Hemagglutinin Glycoproteins, Influenza Virus - chemistry Hemagglutinin Glycoproteins, Influenza Virus - immunology Hemagglutinin Glycoproteins, Influenza Virus - isolation & purification Humans Immunoglobulin Fab Fragments - chemistry Immunoglobulin Fab Fragments - immunology immunosensor influenza A Influenza A Virus, H1N1 Subtype - chemistry Influenza A Virus, H1N1 Subtype - isolation & purification Influenza A Virus, H1N1 Subtype - pathogenicity Influenza, Human - diagnosis Influenza, Human - immunology Influenza, Human - virology Labeling Methods Pandemics Proteins Quenchbody Swine flu Viruses
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Beschreibung
Zusammenfassung:	We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.
ISSN:	1424-8220 1424-8220
DOI:	10.3390/s19010052