CHANGING OF SOME STENOTROPHOMONAS MALTOPHILIA BIOLOGYCAL PROPERTIES UNDER EFFECT OF SODIUM DODECYL SULFATE

Aim: To study biological abilities changing of Stenotrophomonas maltophilia before and after cultivation on the medium with sodium dodecyl sulfate (SDS). Methods. The physiological and biochemical properties, antibiotics sensitivity of S maltophilia were studied due to microbiological analyzer Vitek 2 Compact (Biomerieus, France), cells morphology – light microscopy method (Zeiss Primo Star, Germany) and electron microscopy (Jeol 1400, Japan). Results. The short-time cultivation of S maltophilia (during 1–10 running) in presence of synthetic anionic surfactant SDS in concentration of 50 mg/L that is near minimal inhibition concentration (MIC) results in changing of some biological parameters and properties, e.g. cells became shorter and changed their shape, the different optical density zone were noted in cytoplasm, the dark and transparent inclusion appeared in cells. Some biochemical test results (glutamyl arylamidase, tyrosine arylamidase, urease, citrate (sodium), beta-N-acetyl-galactosidase, L-histidine assimilation, coumarate, 0/129 resistance, L-malate assimilation, Ellman) were changed to opposite index. The MIC of 9 antibiotics increased in 2 times and more, to 4 antibiotics there were formed resistance. Adhesion properties of S. maltophilia varied depending upon run times in presence of SDS. The properties which were changed during the experiment sometimes were restored after the 10th run. This fact could speculate about certain culture adaptation toward to antibiotic. Whereas SDS is the most popular surfactant, which is included in almost all cosmetic and household chemicals, the changes of biological activities of bacteria under its influence could provoke the problems with clinical important strains identification.