Nat1 Deficiency Is Associated with Mitochondrial Dysfunction and Exercise Intolerance in Mice

We recently identified human N-acetyltransferase 2 (NAT2) as an insulin resistance (IR) gene. Here, we examine the cellular mechanism linking NAT2 to IR and find that Nat1 (mouse ortholog of NAT2) is co-regulated with key mitochondrial genes. RNAi-mediated silencing of Nat1 led to mitochondrial dysf...

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Veröffentlicht in:Cell reports (Cambridge) 2016-10, Vol.17 (2), p.527-540
Hauptverfasser: Chennamsetty, Indumathi, Coronado, Michael, Contrepois, Kévin, Keller, Mark P., Carcamo-Orive, Ivan, Sandin, John, Fajardo, Giovanni, Whittle, Andrew J., Fathzadeh, Mohsen, Snyder, Michael, Reaven, Gerald, Attie, Alan D., Bernstein, Daniel, Quertermous, Thomas, Knowles, Joshua W.
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Sprache:eng
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Zusammenfassung:We recently identified human N-acetyltransferase 2 (NAT2) as an insulin resistance (IR) gene. Here, we examine the cellular mechanism linking NAT2 to IR and find that Nat1 (mouse ortholog of NAT2) is co-regulated with key mitochondrial genes. RNAi-mediated silencing of Nat1 led to mitochondrial dysfunction characterized by increased intracellular reactive oxygen species and mitochondrial fragmentation as well as decreased mitochondrial membrane potential, biogenesis, mass, cellular respiration, and ATP generation. These effects were consistent in 3T3-L1 adipocytes, C2C12 myoblasts, and in tissues from Nat1-deficient mice, including white adipose tissue, heart, and skeletal muscle. Nat1-deficient mice had changes in plasma metabolites and lipids consistent with a decreased ability to utilize fats for energy and a decrease in basal metabolic rate and exercise capacity without altered thermogenesis. Collectively, our results suggest that Nat1 deficiency results in mitochondrial dysfunction, which may constitute a mechanistic link between this gene and IR. [Display omitted] •Absence of the insulin resistance gene Nat1 results in mitochondrial dysfunction•Nat1 deficiency in mice is associated with a decreased ability to utilize fats for energy•Nat1-deficient mice have decreased basal metabolic rate and exercise capacity Chennamsetty et al. show that deficiency of Nat1 (ortholog of the human insulin resistance gene NAT2) decreases mitochondrial function in vitro and in vivo. Nat1-deficient mice have decreased basal respiration, maximal exercise capacity, and the ability to utilize fat for energy.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2016.09.005