Emergence of IMP-8-Producing Comamonas thiooxydans Causing Urinary Tract Infection in China

The emergence of carbapenem resistance (CR) caused by hydrolytic enzymes called carbapenemases has become a major concern worldwide. So far, CR genes have been widely detected in various bacteria. However, there is no report of CR gene harboring . We first isolated a strain of an IMP-8-producing from a patient with urinary tract infection in China. Species identity was determined using MALDI-TOF MS analysis and carbapenemase-encoding genes were detected using PCR. The complete genomic sequence of was identified using Illumina Novaseq and Oxford Nanopore PromethION. Antimicrobial susceptibility analysis indicated that the strain ZDHYF418 was susceptible to imipenem, intermediate to meropenem, and was resistant to aztreonam, fluoroquinolones, and aminoglycosides. The gene was chromosomally located, and was part of a Tn402-like class 1 integron characterized by the following structure: . Phylogenetic analysis demonstrated that the closest relative of ZDHYF418 is QYY (accession number: CP053920.1). We detected 330 SNP differences between ZDHYF418 and QYY. Strain QYY was isolated from activated sludge in Jilin province, China in 2015. In summary, we isolated a strain of that is able to produce IMP-8 and a novel . This is the first time that a CR gene has been identified in . The occurrence of the strain needs to be closely monitored.