The association between cervical lesions of different grades and lncRNA HOTTIP and H19 single nucleotide polymorphisms

Background and purpose: Both HOTTIP and H19 are members of long non-coding RNA(lncRNA). They have been found to play an important role in the occurrence, development and migration of tumors. This study aimed to investigate the association between single nucleotide polymorphisms (SNP) of HOTTIP rs2067087, H19 rs2839698 and H19 rs2107425 and the risk of cervical lesions of different grades. Methods: The peripheral venous blood and related clinical data of 345 cases of cervical lesions were collected from Nov. 2018 to Dec. 2020 in Yancheng No.1 People's Hospital, including 220 cases of cervical intraepithelial neoplasia (CIN) and 125 cases of cervical cancer (CC). Three hundred and sixty patients without cervical lesions hospitalized during the same period were selected as the control. TaqMan real-time fluorescence quantitative polymerase chain reaction (RTFQPCR) was used to genotype the HOTTIP SNP rs2067087, H19 SNP rs2839698 and rs2107425 in the two groups, and χ2 test and unconditional logistic regression were used to analyze the association of the three SNP loci with cervical lesions of different grades. Results: Compared with the control group, there were significant differences in genotype distribution of HOTTIP rs2067087 and H19 rs2839698 in patients with cervical lesions. In the recessive model, the frequency of cervical lesions in patients with HOTTIP rs2067087 GG genotype increased by 52.2% (95% CI: 1.021-2.269, P