Development and Validation of a Novel HPLC Method to Analyse Metabolic Reaction Products Catalysed by the CYP3A2 Isoform: In Vitro Inhibition of CYP3A2 Enzyme Activity by Aspirin (Drugs Often Used Together in COVID-19 Treatment)

Aspirin (also known as acetylsalicylic acid) is a drug intended to treat fever, pain, or inflammation. Treatment of moderate to severe cases of COVID-19 using aspirin along with dexamethasone has gained major attention globally in recent times. Thus, the purpose of this study was to use High-Perform...

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Veröffentlicht in:	Molecules (Basel, Switzerland) Switzerland), 2022-01, Vol.27 (3), p.927
Hauptverfasser:	Hussain, Amira, Naughton, Declan P, Barker, James
Format:	Artikel
Sprache:	eng
Schlagworte:	6β-hydroxydexamethasone Acetonitrile Acetylsalicylic acid Animals Aspirin Aspirin - chemistry Aspirin - pharmacology Calibration Chromatography, High Pressure Liquid - methods competitive inhibitor Coronaviruses COVID-19 COVID-19 - drug therapy CYP3A activity Cytochrome P-450 CYP3A - drug effects Cytochrome P-450 CYP3A - metabolism Cytochrome P-450 Enzyme Inhibitors - metabolism Cytochrome P-450 Enzyme System - metabolism cytochrome P450 Dexamethasone Dexamethasone - analogs & derivatives Dexamethasone - pharmacology Drug interaction Drug interactions Drugs Enzymatic activity Enzyme activity Enzymes Experiments Fever High performance liquid chromatography Hydroxylation In vitro methods and tests Liquid chromatography Liver Male Metabolism Metabolites Microsomes Microsomes, Liver - metabolism Nonsteroidal anti-inflammatory drugs Pain Pandemics Pharmaceutical Preparations - metabolism Protein Isoforms - metabolism Rats Rats, Sprague-Dawley Reaction products Reproducibility of Results SARS-CoV-2 - drug effects SARS-CoV-2 - pathogenicity Standard deviation Steroids Toxicity
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Beschreibung
Zusammenfassung:	Aspirin (also known as acetylsalicylic acid) is a drug intended to treat fever, pain, or inflammation. Treatment of moderate to severe cases of COVID-19 using aspirin along with dexamethasone has gained major attention globally in recent times. Thus, the purpose of this study was to use High-Performance Liquid Chromatography (HPLC) to evaluate the in vitro inhibition of CYP3A2 enzyme activity using aspirin in rat liver microsomes (RLMs). In this study, an efficient and sensitive HPLC method was developed using a reversed phase C18 column (X Bridge 4.6 mm × 150 mm, 3.5 µm) at 243 nm using acetonitrile and water (70:30 ). The linearity (r > 0.999), precision (
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules27030927