Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology

Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing tec...

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Veröffentlicht in:	Stem cell research 2024-12, Vol.81, p.103580, Article 103580
Hauptverfasser:	Gong, Tingyu, Liu, Dandan, Wang, Xiaochen, Zhou, Danni, Tang, Ling, Wang, Hao, Su, Jun, Liang, Ping
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Cell Line CRISPR-Cas Systems Gene Editing - methods Gene Knockout Techniques - methods Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism
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Beschreibung
Zusammenfassung:	Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.
ISSN:	1873-5061 1876-7753 1876-7753
DOI:	10.1016/j.scr.2024.103580