Normal-phase high performance liquid chromatography of estradiol derivatives on amino- and diol-columns

The retention behavior of estradiol derivatives was studied by HPLC on chemically bonded polar stationary phases: commercially available amino and diol- columns, as a function of the heptane-propan-1-ol as the mobile phase, when the volume fraction of propan-1-ol in the binary mobile phase was low, even less than 5 %. The relationship between the logarithm of the retention constant (log k) and the logarithm of the volume fraction of propan-1-ol (?log ?) in the eluent was linear for all solutes studied. The results are discussed in terms of the solute and stationary phase properties and compared with the results of the same derivatives obtained in earlier investigations. U radu je primenom visoko pritisne tecne hromatografije ispitano retenciono ponasanje derivata estradiola na amino- i diol- hemijski vezanoj fazi. Kao pokretna faza koriscena je smesa heptan?1-propanola, u razlicitim odnosima. Udeo 1-propanola bio je nizak, u nekim pokretnim fazama manji od 5 %. Retenciono ponasanje ispitivanih derivata je diskutovano sa aspekta prirode rastvorka, nepokretne i pokretne faze. Za sve ispitane derivate dobijena je linearna zavisnost izmedju logaritma retencione konstante i logaritma zapreminskog udela polarnije komponente pokretne faze tj., 1-propanola. Iako su retenciona vremena derivata estradiola duza na amino koloni, efikasnost razdvajanja na razlicitim kolonama je veoma slicna. Dobijeni rezultati su uporedjeni i sa retencionim podacima istih jedinjenja dobijenih u ranijim ispitivanjima na silika gelu.