CircMAPK9 promotes adipogenesis through modulating hsa-miR-1322/FTO axis in obesity

Circular RNA (circRNA) is a special category of non-coding RNA that has garnered increasing attention in the exploration of lipid metabolism. However, the functional regulation mechanisms of circRNAs in obesity diseases remain unclear. By whole transcriptome sequencing, a total of 164 circular RNAs were found to exhibit differential expression between lean and obese individuals. RT-qPCR was used to detect significant expression of circMAPK9 in obese individuals, and it was closely related to BMI. Western blot, triglyceride detection, and Oil Red O staining were employed to investigate the role of circMAPK9/hsa-miR-1322/FTO in adipogenesis. In adipocytes, the connection between hsa-miR-1322 and circMAPK9 was verified using fluorescence in situ hybridization, luciferase reporter assay, and RNA immunoprecipitation. It was found that circMAPK9 competed for binding hsa-miR-1322 in the cytoplasm, weakening the inhibitory effect on FTO and promoting adipogenesis. Our study revealed the regulatory mechanism and important role of circMAPK9 in the process of adipogenesis. [Display omitted] •CircMAPK9 expression level was significantly correlated with BMI in obese patients•CircMAPK9 is involved in adipogenesis and acts as a key regulator•CircMAPK9 sponges hsa-miR-1322 to regulate FTO expression through a ceRNA mechanism Molecular biology experimental approach; Molecular network; Specialized functions of cells; Transcriptomics