Genomic Analysis of Sphingopyxis sp. USTB-05 for Biodegrading Cyanobacterial Hepatotoxins

sp. USTB-05, which we previously identified and examined, is a well-known bacterial strain for biodegrading cyanobacterial hepatotoxins of both nodularins (NODs) and microcystins (MCs). Although the pathways for biodegrading the different types of [D-Asp ] NOD, MC-YR, MC-LR and MC-RR by sp. USTB-05 were suggested, and several biodegradation genes were successfully cloned and expressed, the comprehensive genomic analysis of sp. USTB-05 was not reported. Here, based on second and third generation sequencing technology, we analyzed the whole genome of sp. USTB-05, which is 4,679,489 bp and contains 4,312 protein coding genes. There are 88 protein-coding genes related to the NODs and MCs biodegradation, of which 16 genes ( , , , , , , , , , , , , , , and ) are unique. These genes for the transformation of phenylacetic acid CoA (PA-CoA) to CO were also found in sp. USTB-05. This study expands the understanding of the pathway for complete biodegradation of cyanobacterial hepatotoxins by sp. USTB-05.